OncoForma's patient-derived organoid platform provides functional drug sensitivity data to guide precision therapy selection

Overview of the Platform

OncoForma has developed a clinical-grade organoid testing platform that cultures patient tumor cells in 3D conditions that closely mimic the native tumor microenvironment. By testing multiple cancer therapies directly on living patient tumor cells, we provide oncologists with personalized drug sensitivity data to inform treatment decisions.

Key Platform Features

3D Organoid Culture

Patient tumor cells are cultured in 3D extracellular matrix conditions that preserve tumor architecture, cell-cell interactions, and heterogeneity.

High-Throughput Screening

Automated drug screening platform tests 25-50 cancer therapies simultaneously, including chemotherapy, targeted agents, and immunotherapy.

Functional Readout

Direct measurement of tumor cell viability, apoptosis, and drug-induced cytotoxicity provides functional sensitivity data.

Clinical-Grade QC

Rigorous quality control ensures organoid viability, genetic concordance with source tumor, and reproducibility of drug response data.

Core Methodology

1. Patient-Derived Organoid (PDO) Culture

  • Fresh tumor tissue from biopsy or resection
  • Enzymatic dissociation and 3D culture in special matrix
  • Organoids should maintain:
    • Original tumor heterogeneity
    • Histological architecture

2. High-Throughput Drug Screening

  • 1,800+ compound library including:
    • FDA-approved drugs
    • Targeted therapies (kinase inhibitors)
    • Emerging therapeutics in clinical trials
  • Automated liquid handling for reproducibility
  • Real-time imaging and analysis

3. Viability and Response Assessment

  • ATP-based luminescence assays (CellTiter-Glo 3D)
  • Live/dead imaging
  • Dose-response curve generation
  • IC50 and AUC calculations for top compound

4. Genomic Integration

  • Whole-exome sequencing
  • RNA expression profiling (when indicated)
  • Correlation of functional responses with molecular features

5. Clinical Report Generation

  • Drug sensitivity ranking (highly sensitive/moderate/resistant)
  • Comparison to standard-of-care regimens
  • Alternative treatment recommendations
  • Literature support and clinical trial options

Timeline & Turnaround

Step Timeline Details
Sample Collection & Shipping Day 0-1 Sample collected by oncologist/surgeon and shipped same day
Sample Receipt & Processing Day 1-2 Sample received at OncoForma lab, processed, and organoid culture initiated
Organoid Establishment & QC Day 3-14 Organoids expand to sufficient numbers; viability and genetic validation performed
Drug Screening Day 14-21 High-throughput drug exposure and viability measurement
Data Analysis & Report Day 21-25 Bioinformatics analysis, medical interpretation, report generation
Total Turnaround 21-25 days From sample receipt to report delivery

Expedited Processing: For urgent clinical scenarios (rapidly progressing disease, limited treatment options), expedited 14-18 day turnaround may be available upon request. Please discuss with our clinical team.

Clinical Validation & Accuracy

OncoForma's platform has been extensively validated in clinical studies demonstrating correlation between organoid drug sensitivity and patient response:

Metric Performance Clinical Significance
Positive Predictive Value 87-92% Patients whose tumors are sensitive in organoids have high likelihood of clinical response
Negative Predictive Value 89-94% Patients whose tumors are resistant in organoids are unlikely to respond
Concordance with Clinical Outcome 78-85% Overall agreement between organoid prediction and actual patient response
Culture Success Rate 75-85% Percentage of samples successfully generating viable organoids for testing

These validation metrics are derived from prospective clinical studies in colorectal cancer, gastric cancer, pancreatic cancer, and ovarian cancer. Full study details are available on our Clinical Evidence page.

Advantages Over Standard Molecular Testing

Feature OncoForma Organoid Testing Standard Genomic Testing
Information Provided Functional drug sensitivity across 25-50 drugs Genetic mutations and potential targets
Predictive for Chemotherapy ✓ Direct testing of chemotherapy agents ✗ Limited utility for chemotherapy selection
Captures Tumor Heterogeneity ✓ Tests all viable tumor cell populations Partial (depends on sequencing depth)
Identifies Resistance Mechanisms ✓ Functional resistance measured directly Only if resistance mutation is known
Actionable for Patients Without Targetable Mutations ✓ Provides guidance for ~95% of patients ✗ Only ~15-25% have actionable mutations
Turnaround Time 21-25 days 4-6 weeks

Complementary Approach: OncoForma testing is designed to complement, not replace, standard molecular profiling. We recommend both genomic testing and organoid functional testing to provide comprehensive information for treatment planning.

Next Steps for Oncologists

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